本剧集场景产生在佛罗里达州的迈阿密，以本地办案人员为故事主轴，描述他们是如何地透过高科技的帮助，以及巨细靡遗的传统办案伎俩，破解一个又一个待解的刑案。附属于迈阿密犯法鉴证科的犯法现场观察小组，频频收支种种案发明场寻找证据和蛛丝马迹，小组成员应用最先进的科技手腕，把外貌上没有任何意义的线索拼凑成不可反驳的证据，抽丝剥茧重组案情，如《犯法现场观察第三季》剧情那样让犯法分子无处可逃……CSI:CrimeSceneInvestigationtooktelevisionbystormin2000withitsinnovativeandcutting-edgeupdatingofastandardseriesconcept–inthiscase,thecopshow.Suddenly,insteadofsimplywhodunit,solvingcrimeswasamatterofhowdunit,andwithwhatsizeshoe,andwhetheranyoneleftbehindtracesofevidencethattheblueALSlightcouldpickup.NowthatgrittyapproachtocrimeknownasforensicsciencemakesitswayfromLasVegastothestreetsofFloridainCSI:Miami.AwholenewteamofcrimesceneinvestigatorsisonhandtoprobetheseamyundersideofwhatsomerefertoasCubaNorthandotherscallGeorgiaSouth.Consideritatropicalblendofpeople,culture,nightlifeand,ofcourse,theoccasionalkillingortwo.JustasyoudoinVegas,comeforthecrime,stickaroundfortheclues.Andthere"salwaysaclue.
